Innovation of the protocol for the application of cryolipolysis: Effects and mechanisms of action
Abstract
Background: In recent years, aesthetic procedures aiming at body remodeling and
have grown exponentially. Cryolipolysis (CLL) has stood out as a noninvasive resource
that acts directly on the subcutaneous adipose tissue promoting a significant reduction of adipose tissue through of cooling that could lead to the crystallization cytoplasmic lipids, loss of cellular integrity, apoptosis/necrosis of adipocytes, and local
inflammation, producing selective loss of adipose tissue. Thus, the objective of the
present study was to evaluate the effects of a specific technique of CLL application
on the inflammatory reactions of the target tissue in different post-application times.
Methods: This is a randomized, blind clinical study that evaluated the tissue sample
of six patients after 45, 60, and 90?days of an innovative protocol for the application
of CLL, with samples collected through abdominoplasty surgeries. The samples were
evaluated by immunohistochemical analyses of several markers.
Results: A significantly greater increase in fibroblasts was observed at 45?days and
greater phagocytic action at 60?days.
Regarding the apoptosis process, the expression of caspase 3 and cleaved caspase
3 markers varied at different times, with cleaved caspase 3 being higher at 45 and
90?days after CLL application.
Conclusion: The protocol of the CLL presented in this study was able to induce inflammatory responses in addition to confirming the selective apoptotic action at the
different times studied.
Abstract
Background: In recent years, aesthetic procedures aiming at body remodeling and
have grown exponentially. Cryolipolysis (CLL) has stood out as a noninvasive resource
that acts directly on the subcutaneous adipose tissue promoting a significant reduction of adipose tissue through of cooling that could lead to the crystallization cytoplasmic lipids, loss of cellular integrity, apoptosis/necrosis of adipocytes, and local
inflammation, producing selective loss of adipose tissue. Thus, the objective of the
present study was to evaluate the effects of a specific technique of CLL application
on the inflammatory reactions of the target tissue in different post-application times.
Methods: This is a randomized, blind clinical study that evaluated the tissue sample
of six patients after 45, 60, and 90?days of an innovative protocol for the application
of CLL, with samples collected through abdominoplasty surgeries. The samples were
evaluated by immunohistochemical analyses of several markers.
Results: A significantly greater increase in fibroblasts was observed at 45?days and
greater phagocytic action at 60?days.
Regarding the apoptosis process, the expression of caspase 3 and cleaved caspase
3 markers varied at different times, with cleaved caspase 3 being higher at 45 and
90?days after CLL application.
Conclusion: The protocol of the CLL presented in this study was able to induce inflammatory responses in addition to confirming the selective apoptotic action at the
different times studied.